Clayton Trevino

5 Questions 3 Answers 0 Followers

Questions related from Clayton Trevino

Antibodies for targeting CRF1 and CRF2?

Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?

24 July 2017 8,957 1 View

QPCR Cost Analysis

I'm doing a cost analysis for a PCR experiment and want to make sure I have left anything out that I may need. The list is currently as follows: Primers, TRIzol Reagent, RNAse Free Water, RNAse...

10 July 2017 4,922 4 View

Does anyone know of a good housekeeping gene for gfap and/or Glt1 expression?

In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.

03 July 2017 652 4 View

What RNA isolation kit would you recommend I use in isolating RNA from brain tissue?

I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.

30 June 2017 8,428 3 View

How much tissue can I expect to get from a VTA dissection of mice brain?

I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...

30 June 2017 3,786 3 View