Hello everyone, could you guys please help me to decide what antibodies should I use to do FACs on Bone marrow derived macrophages from my mice model to check specific types of macrophages( like M1, M2, M2a, M2b etc) the BMDM turn in to ?
Dear Antara!
Please You look at the articles:
Article Investigation of Macrophage Polarization Using Bone Marrow D...
Figure 3. Macrophage polarization analysis. Tissue infiltrated macrophages are defined as CD11b+F4/80+ cells; M1 macrophages are CD11b+F4/80+CD11c+CD206- cells, whereas M2 macrophages are CD11b+F4/80+CD11c-CD206+ cells.
Figure 4. Macrophage activation analysis by flow cytometry.(A) Upon activation, the size of macrophages increase as evidenced by the shift of FSC-A of M1 or M2 macrophages at 24 hr after stimulation compared to M0 (untreated) macrophages. (B) The activation-related surface markers were analyzed by flow cytometry after IL4 or LPS stimulation. The early responding marker CD69 was evaluated at 5 or 24 hr post-stimulation; CD80 and CD86 markers were analyzed at 48 hr after stimulation.
Article Acute myeloid leukemia cells polarize macrophages towards a ...
Figure 4.
Figure 5.
Hi! I have been through this issue before and found this mini-review by Bio-Rad which I have found very useful.
https://www.bio-rad-antibodies.com/macrophage-polarization-minireview.html
Table 1 is a great guide for Flow Cytometry but check out the other tables which also help you through other kinds of markers as well.
Regards!!
We used this protocol for a monocyte study in TB patients. Hope it helps
Preprint Lower Monocyte Interleukin-7 Receptor Expression Impairs Ant...
Thank you so much Alexandr Chernov , Amy Mónaco and Wilfred Aniagyei , my experiment got delayed and could not work on FACS, will start working on it next week and will use every information that you guys have helped me with. Thank you all again :)