Antibodies for Cerulean/CFP that do not cross-react with GFP?

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Any cell-cycle marker antibodies for zebrafish except PCNA and Ki67?

Hi all, I am working on the enteric nervous system of zebrafish and I am looking for a cell-cycle marker to label proliferating cells. I have tried antibodies against Ki67 (NCL-Ki67p and 550609)...

31 December 2019 6,878 3 View

Why did my mice die after stereotaxic surgery?

I performed yesterday 6 subretinal injections using Isofurane as an anesthetic. The surgery lasted something less than an hour, isoflurane was set to 2% and mice were treated with Metamizol and...

03 April 2018 4,153 3 View

Tips for better titration of AAV2?

Hi researchgate community. I got a question for you. I would like to purify AAV from HEK293T cells and I am trying to enhance my titer from 10^9 to 10^12. Any tips or highlights? Thank you!!

01 February 2018 9,868 5 View

Working antibody for staining glycinergic neurons in the mouse spinal cord?

Hi everyone! I have a question regarding staining glycinergic neurons in the spinal cord of Bl6 mice. The literature is kind of limited on this topic. I want to stain these neurons, I was thinking...

09 October 2016 5,400 3 View

Staining mouse spinal cord with parvalbumin in far red not working?

Hey everyone, i am trying here to stain mouse spinal cord with parvalbumin(mouse origin), from swant, dilution 1:5000 with alexa fluor 647 or 633. The problem is that that the staining is very...

06 July 2016 7,501 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View