Evangelia Fourli

6 Questions 8 Answers 0 Followers

Questions related from Evangelia Fourli

Any cell-cycle marker antibodies for zebrafish except PCNA and Ki67?

Hi all, I am working on the enteric nervous system of zebrafish and I am looking for a cell-cycle marker to label proliferating cells. I have tried antibodies against Ki67 (NCL-Ki67p and 550609)...

01 January 2020 6,799 3 View

Antibodies for Cerulean/CFP that do not cross-react with GFP?

Hello all! I know this is a tricky question and most likely no such antibody exists but I wanted to try my chances in case any of you has used an anti-CFP antibody with no cross reactivity to...

08 August 2019 8,419 4 View

Why did my mice die after stereotaxic surgery?

I performed yesterday 6 subretinal injections using Isofurane as an anesthetic. The surgery lasted something less than an hour, isoflurane was set to 2% and mice were treated with Metamizol and...

04 April 2018 4,098 3 View

Tips for better titration of AAV2?

Hi researchgate community. I got a question for you. I would like to purify AAV from HEK293T cells and I am trying to enhance my titer from 10^9 to 10^12. Any tips or highlights? Thank you!!

02 February 2018 9,805 5 View

Working antibody for staining glycinergic neurons in the mouse spinal cord?

Hi everyone! I have a question regarding staining glycinergic neurons in the spinal cord of Bl6 mice. The literature is kind of limited on this topic. I want to stain these neurons, I was thinking...

10 October 2016 5,335 3 View

Staining mouse spinal cord with parvalbumin in far red not working?

Hey everyone, i am trying here to stain mouse spinal cord with parvalbumin(mouse origin), from swant, dilution 1:5000 with alexa fluor 647 or 633. The problem is that that the staining is very...

07 July 2016 7,440 3 View