Hi , Dear member, I m doing m.sc project (6 months) in title " The in vitro Antibiofilm activity of selected medicinal plants against candida albicans, staphylococcus , streptococcus, P. areginosa ". I need ur suggestion. On what basis (or which medicinal property) , I should select the medicinal plants for checking antibiofilm activity ? My another question is "Is neccessary to check antimicrobial property before antibiofilm activity"?
Hello Mahesh, good to know about ur plan. First u check with the medicinal plants which people use and check whether some one have made a study on them. collect research pdf or lit regarding ur project. see what techniques, hw far they carried out their work. some would hv stopped with just antimicrobial activity u can carry out rest of the work like Hplc, GC-MS. or u can even select a untouched plant parts and find the active phytochemical structure which bind with the microb in wet lab or by using bioinfo tool. try to study MIC against these microbs. use any solvent extract. i think this will help u. All the very best.
Biofilm study works out well wen study on many organism live together. Pseudomonas aeruginosa is a well know organism for its biofilm. try to fig out some physics calculation as the study depends on the surface tension. search for article n goodle search, pubget, pubmed, nature etc. here is a example.
Performing your original search, antibioflm activity of medicinal plants, in PubMed will retrieve 10920 records.
Lett Appl Microbiol. 2010 Jan;50(1):30-5.
The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes.
Sandasi M, Leonard CM, Viljoen AM.
Source
Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa.
Abstract
AIMS:
The antibiofilm activity of extracts obtained from selected herbs, spices, beverages and commercially important medicinal plants was investigated on Listeria monocytogenes.
METHODS AND RESULTS:
The growth and development of the biofilm was assessed using the crystal violet (CV) assay. The respiratory activity was assessed using the 2, 3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) reduction assay. The majority of extracts tested prevented cell adhesion to the polyvinyl chloride (PVC) surface. Seven of the 15 extracts reduced biofilm adhesion of both the clinical and the type strains by at least 50%. In contrast, inhibition of a preformed biofilm was more difficult to achieve, with only three extracts (Rosmarinus officinalis, Mentha piperita and Melaleuca alternifolia) inhibiting the growth of both strains by at least 50%.
CONCLUSIONS:
Although most extracts were able to reduce initial cell attachment, inhibition of growth in a preformed biofilm was more difficult to achieve.
SIGNIFICANCE AND IMPACT OF THE STUDY:
The ability to reduce biofilm biomass as shown by several plant extracts warrants further investigation to explore the use of natural products in antibiofilm adhesion.
PMID: 19874481 [PubMed - indexed for MEDLINE]
MeSH Terms, Substances
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MIC will help u and give u hope tat ur plant extract inhibit the microbs growth. then u can carry out the antibiofilm effect example by microtiter plate assay.
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