How to suppress model part for any specific step in Abaqus/explicit analysis without using deactive/suppress option using load manager option?

I am doing a Abaqus/explicit dynamic analysis. The moving system that I am using as a impact body need to be active in a final analysis step. I'm providing velocity of moving body using...

02 April 2018 1,169 3 View

What are the difference between providing velocity using predefined field and using boundry conditions in Abaqus?

Hi all, i am doing dynamic impact analysis on a civil structure. And the impact load is a moving load with a certain magnitude of velocity. And i have to analyse the effect of impacting body in...

18 March 2018 8,361 5 View

How to plot predicted probability of logistic regression with multiple variables?

I encountered a problem in plotting the predicted probability of multiple logistic regression over a single variables. For example, my model is Prob = - 0.727 + -0.002*X1+ -0.022*X2+ -0.002*X3+...

20 December 2017 5,725 11 View

Does anyone know how to separate supraparamagnetic nanoparticles (20-50nm core, produced by Turbobeads) that have formed small self-aggregations?

The product will not stay in a uniform suspension, aggregates visible under the microscope. These are for in vivo use.

11 December 2017 375 2 View

Is there any JCPDS card / XRD details for Cellulose, Hemicellulose, Lignin and other components for natural fibers?

i am working on natural fibers, and in their XRD pattern i am getting 2 peaks. First is at 15.822 degree and second is at 22.1503 degree of 2 theta. yet the intensity is low after convolution (80...

11 July 2016 2,048 5 View

How do I evaluate the performance of a network in MATLAB?

20 March 2015 6,171 6 View

What is best validation performance in artificial neural network?

01 December 2014 9,296 4 View

I am having trouble 'mating' (also called conjugation) a 70,000 bp DNA fragment from E. coli. into Streptomyces - can anyone help?

I am trying to transfer a 70,000bp secondary-metabolite-producing piece of DNA from E coli. 12567 cells into Stretpomyces Albus and Amycolatopsis Balhimycina by mating/conjugating them on an ISP4...

12 November 2013 5,492 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View