Hi
I am trying to show that a compound induces translocation of glut4 in primary porcine muscle cells. I am working on blebbing giant membrane vesicles which I would like to immunostain for glut4. However, I will not be able to permeabilize the vesicles, and therefore need an antibody (commercial) that binds to an extracellular epitope on glut4 (if such an ab exists), and will react with pig glut4.
Does anybody know of any ab that might work for this?
I have been checking ab's, but there are quite many for glut4, and I haven't been able to find one yet. I can not transfect these cells, as they are quite delicate to work with.
I just wanted to ask, I could have been lucky that someone had this problem before me.
Oh well, I guess I will just have to continue searching.
Thanks, looks promising. I can't believe I missed that, I was sure I searched their site ;-)
I found one at US Biological also, but have bought from santa cruz before, so I think I will check that one out.
Antibodies against the GLUT4 epitope are indeed problematic. I am also unable to find any antibodies (no matter the species) for FACS. Someone told me they get shedded from the membrane and that is why they are unavailable.
Anybody know why there are no/ very hard to find antibodies against it?
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