Annealing and extending oligos

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Gamal Abdelaziz

you have to make PCR for your product you want , also more useful to make small prier for you original one

Aamal Ghazi Mahdi Al-Saadi

https://www.neb.com/tools-and-resources/usage-guidelines/guidelines-for-pcr-optimization-with-taq-dna-polymerase

http://www.sigmaaldrich.com/technical-documents/protocols/biology/annealing-oligos.html

Niradiz Reyes

IDT can synthesize duplex oligos. It may be practical to order dsOligos from a dedicated company. They are cheap.

Yuan-Yeu Yau

If the sequences of the two oligomers are complement, then people just heat them (mixed solution) up in water or in buffer and then cool it down to room temperature. You should get dsOligo DNA fragment that way.

See here for one example: " Annealing oligonucleotides " [https://www.idtdna.com/pages/decoded/decoded-articles/pipet-tips/decoded/2012/06/15/annealing-oligonucleotides]