6 Questions 10 Answers 0 Followers
Questions related from Lisandro Alfonso Pacheco
Anybody has a working protocol to anneal and extend ss oligos to get a dsDNA using Taq DNA polymerase?
12 May 2017 1,037 4 View
Hi everybody. Anybody knows what is the meaning of the number "36,04 g/mol" in the NEB's formula to calculate molarity of dsDNA ends?. Link: http://nebiocalculator.neb.com/#!/dsdnaends Thanks in...
20 October 2015 7,389 2 View
I have two complementary sequences of DNA and I need to form a duplex. Each sequence have 61 nuecleotides. Can you help with a protocol? Thank you.
20 March 2015 9,367 4 View
Sticky ends to blunt ends using DNA pol I?
04 March 2015 8,188 5 View
I want to profile miRNA's in plasma of patients with lupus nephritis through Next Generation Sequencing. I have been reading about the advantages both Illumina and 454 in RNA-seq.
05 December 2012 6,282 8 View
Hi everybody. I usually estimate the cell confluency in culture flasks/plates in a subjective way, looking the cells and then calculating what is the area they are populating. ¿Do exist a...
13 February 2012 4,621 17 View
