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Anybody has a working protocol to anneal and extend ss oligos to get a dsDNA using Taq DNA polymerase?
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I have two complementary sequences of DNA and I need to form a duplex. Each sequence have 61 nuecleotides. Can you help with a protocol? Thank you.
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Sticky ends to blunt ends using DNA pol I?
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I want to profile miRNA's in plasma of patients with lupus nephritis through Next Generation Sequencing. I have been reading about the advantages both Illumina and 454 in RNA-seq.
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Hi everybody. I usually estimate the cell confluency in culture flasks/plates in a subjective way, looking the cells and then calculating what is the area they are populating. ¿Do exist a...
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