Hi,

I am doing an experiment with S. pombe. I want to show that the enzyme I transformed into S. pombe can "rescue" the phenotype of the KO strain which has a longer S phase. My growth curve accepted the hypothesis, but I want some more specific evidence. I want to compare the KO strain and the transformed strain, and show transformed strain has a shorter S phase. I want something that can stain DNA in the cells, take pictures under the microscope, and then analyze the picture to see how many cells are in the S phase. If more cells in KO strains are in the S phase, it will accept my hypothesis.

Our lab is not a yeast lab and we are not super familiar with fission yeast experiments. When I am reading literature, it seems like most studies about cell cycle use flow-cytometry. I wonder if the flow-cytometry is the gold standard to show cell cycle? Or my thought is applicable as well?

Thank you very much!

Best regards,

Heng