Could anyone help me with a SNP primer desing?

16 February 2021 3,049 3 View

Can you share your online teaching-learning experience during this pandemic period?

I have started online classes for undergrad, master, and Ph.D. students last January 16 up to the end of May. I have request students to allow recording classes and to discuss the process at the...

14 September 2020 4,466 23 View

Does someone have a protocol for glucose uptake experiments in vivo?

I am planning to run a glucose uptake experiment in mice (In vivo). I would like to inject H3-2DG and just measure the percent of H3 in different tissues. No clamp experiment, but I was thinkMing...

18 February 2020 9,531 3 View

Can I use different rotation tecniques to compare extract factors in different years?

Dear researchers, I obtained a rotation matrix (varimax) in my factor analysis with correlation no higher than 0.20 for my 2010 data. However, for my 2014 data, with varimax rotation, I obtained...

20 January 2020 5,393 2 View

Isolating high molecular weight DNA from a single feather?

I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...

14 August 2018 5,190 2 View

Does anyone know of a methodology to quantify the enzymatic activity of CYP7B1 (oxysterol 7-alpha-hydroxylase) in serum / plasma?

As for example GC-MS (gas chromatography coupled to mass spectrometry).

12 March 2017 8,803 3 View

Can I store genomic DNA at -20 C for a year without degradation that could affect qPCR runs by that time?

Hi, I will need to run qPCR on genomic DNA samples in about a year from now. Right now isolated DNA is preserved at -20 C. Does anyone know if I should do something else with the samples so that...

30 November 2016 8,366 6 View

Does anybody know if the Sysmex XT-2000i analyser can be connected to LIMS system?

14 January 2015 1,416 2 View

Is there any specific RNAse for short dsRNA?

18 November 2014 1,038 1 View

How to check siRNA molecule thermodynamics?

I've been questioned about the thermodynamics of my siRNAs molecules. It is well described that the strand with less stability at 5' has a higher probability to be incorporated at RISC complex....

18 May 2014 5,472 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View