Hello,
1. Any idea about the HPLC/MS method followed for analysis and reporting of the 10 mercapturic acids in G-EQUAS PTs (https://app.g-equas.de/web/).
2. Also they have two PT urine samples (PT A and PT B), with one usually in lower concentration range and other in higher concentration range of occupational exposure. Any idea if they are spiked urines samples or real-pooled urine samples ?
Dear Vineet Kumar Pal,
The following article could be useful. Good luck.
Thompson, C. D., Gulden, P. H., & Macdonald, T. L. (1997). Identification of modified atropaldehyde mercapturic acids in rat and human urine after felbamate administration. Chemical research in toxicology, 10(4), 457-462.
Following article will be useful for the first part of your question...
1. Jones AD, Winter CK, Buonarati MH, Segall HJ. Analysis of mercapturic acid conjugates of xenobiotic compounds using negative ionization and tandem mass spectrometry. Biol Mass Spectrom. 1993 Jan;22(1):68-76. doi: 10.1002/bms.1200220109. PMID: 8431504.
2.Al Janobi AA, Mithen RF, Gasper AV, Shaw PN, Middleton RJ, Ortori CA, Barrett DA. Quantitative measurement of sulforaphane, iberin and their mercapturic acid pathway metabolites in human plasma and urine using liquid chromatography-tandem electrospray ionisation mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Dec 5;844(2):223-34. doi: 10.1016/j.jchromb.2006.07.007. Epub 2006 Aug 23. PMID: 16931178.
Good luck.......
Mr. Pal,
Currently, among the developed quantitative mass spectrometric approaches, worldwide, there can be achieved exact and reliable analytical information, in chemometric terms, by means of mass spectrometry, only via our own authored (mine and my co-author's according to the authoreship shown in references [1-3], below) innovative stochastic dynamic theory and model formulas [1,2].
[1] Analytical Chemistry Letters, 10 (2020) 703-721; Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller.
[2] Steroids, 164 (2020) 108750 Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller.
Only, this theory accounts exactly for the fluctuations of the measurable variables of intensity data on analytes. For this reason, correlative analysis between intensity values according to our model equations and analyte concentration in solution at pg.(L)-1 and ng.(mL)-1 levels of mixtures yields to excellent-to-exact statistical parameters (/r/ = 0.99997-1.)
The employment of the classical approach to quantify analytes by means of total intensity value (ITOT, arb.units) over the whole time of a measurement could, or could not provide reliable results. For instance, consider some still unpublished data on analysis of steroids showing that relationships ITOT = f(conc.) yield to /r/ = 0.94-0.99951 (Attachment RGFig1.gif, [3].)
[3] B. Ivanova, M. Spiteller, Quantitative analytical aspects of the 3D mass spectrometric structural determination based on stochastic dynamics – analysis of steroids (part III) (2021/2022) in preparation.
- Badges
- Science topic
- Similar topics
- Chemistry, Organic
- Organic