Vinayak Ghosh

3 Questions 1 Answers 0 Followers

Questions related from Vinayak Ghosh

Analysis for Spatial Distribution?

I am doing an experiment where I see in IF that the localization of the protein is such that it gets dispersed all over the cell from just being perinuclear. How would I perform the analysis in...

23 June 2023 2,525 0 View

For BioID experiments, is it okay to use Streptavidin - Agarose instead of Streptavidin-Sepharose for pulldown?

For most experiments, people use Streptavidin Sepharose beads (affinity of >= 300nm free biotin) (GE Healthcare). However, we would like to use Streptavidin Agarose (affinity of >=120nm free...

05 July 2021 4,904 1 View

What is the exact protocol of ROS measurement using DCFDA?

I am trying to measure ROS production in HT22 cell line post 24 hours of treatment in 24 well plate. The two protocols which I have found: 1. post 24 hour of treatment to incubate 5uM of DCFDA by...

21 October 2020 4,433 5 View