Dear Sir,

The best way to overcome the multiple band is high annealing temperature, if reported temperature is about 55C and you got multiple bands then used higher temperatures up to 60C. The fast way to do this is use of gradient PCR but if you still face same problem then only one thing left which is the primer sequences. You can Blast primer sequences and check if it showed similarity in many time in a same strain or genus especially sequence at start and end then replace these sequences or designed primers again. May it will help