I am culturing RAW 264.7 cell line but I am frequently observing infection. So among this Plasmocin and Normocin which one shoul I use and in what dose? Please suggest me
Ok. I will try to follow that
What is the specific cucumber (Cucumis sativus) biomarker for standerdization and fingerprinting using HPLC.
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Hybrid composite matrix -Aluminium Alloy Reinforcement - Egg shell and SiC
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As an antioxidant role of zinc, conflicting reports are pouring. It is recognized that Zn has prominent role in reducing oxidative stress by scavenging and decreasing production of ROS. On the...
19 June 2023 9,769 1 View
I want to isolate tRNA from adherent cells (DLD1) which will be used for further screening assays. is there any kit available or any protocol that I need to follow? Its a bit urgent. Thanks in...
03 March 2023 3,084 1 View
Usually there are cDNA synthesis kit for microRNA but not for piRNA , previously we were using miScript RT II kit , but the product has been discontinued for now .If anyone can suggest any ideas.
08 January 2023 1,743 6 View
I am trying to express gamma-synuclein using E. coli BL21 cells. Antibiotic conc: 50mg/ml, IPTG 1mM for 4 hrs, 37 degrees. After sonication, boiling water bath is used before cell lysate...
27 June 2022 8,638 1 View
Should we use a single goodness of fit measure like R square for evaluating performance of multiple regression application or a number of measures like R-square, adj R square, AIC, BIC, RMSE etc....
20 October 2021 3,249 5 View
Can any body give any open source links for downloading stock prices of important global hospitality firms and restaurants? The data should be daily/weekly stock price from August 2019 till...
01 October 2021 9,689 2 View
Development of bioconcrete material using an enrichment culture of novel thermophilic anaerobic bacteria May 2006 Indian Journal of Experimental Biology 44(4):336-9 P Ghosh, Saroj Mandal. S Pal, G...
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Can anybody share opensource Partial Least Squares (PLS) tools that is easy to handle, reliable and easy to learn analysis and interpretation?
16 September 2021 9,692 6 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
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What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
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Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
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Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
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As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View