I have been doing an iodine binding assay for my branching enzyme and done a time course. However, the master mix i created finished at a 4
hour mark. I created a new master mix and will wait for it to reach 7 hour time point. Is this method causing manipulation of results?
Dear Syed,
changing to a different batch of master mix could likely introduce a greater variablility in your results, which could make them more difficult to interpret.
I would always recommend to calculate the necessary amounts of reagents prior to the start of the experiment and use the same buffer/ master mix preparations throughout. If your changes are small, variations in component concentrations due to normal pipetting errors can have an impact on your results.
Best,
Marianne
you can read the literature and then you is starting the work, didn't find any work without errors, go a head
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