Alternatives to PicoGreen for dsDNA in non-pure samples?

02 May 2023 1 1K Report

I'm using PicoGreen to quantify dsDNA concentration in samples containing proteins, enzymes, and salts.

In troubleshooting, I found that the individual enzymes and salts were giving a strong false positive signal from the PicoGreen.

I had the same issue when trying dsDNA quantification with spectrophotometry, since the other peaks interfered with the signal.

Does anyone know if there is an alternative to PicoGreen which is less sensitive to contaminants?

Péter Gyarmati

I cannot think of any DNA measurement technique that would not be sensitive to protein and/or salt contamination. Cant you purify a few ul of your sample and measure DNA from that?

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