DMAE (deanol bitartrate)- dimethylaminoethanol bitartrate?

Hello all... I m looking for a method for assay DMAE (deanol bitartrate)- dimethylaminoethanol bitartrate in aqueous samples (cosmetic product) using GC- MS mass spectrum HP- 5 ms U.I 0.25...

13 February 2021 7,629 3 View

Can anyone suggest a PEG replacement in toothpaste formulations?

I am aware that PEG has unique properties however I am just reaching out there to see if anyone can suggest a valuable replacement. I was thinking Na PCA. Thank you

16 March 2020 2,729 3 View

Mudvolcanoes, fractures, gas and thermal water. Which are the relationships between these in pre-Apennines areas (central italy)?

Are Mudvolcanoes linked to rep thermal water? And, which are their connections with sinkholes areas?

25 September 2019 8,864 3 View

Why did you publish our MN without our pemission?

Dear Sir/Madam, this is an e-mail that I am going to send to your attention. It is mandatory that you cancel from your web site our manuscript submitted to the journal Cells. The manuscript...

16 April 2019 1,611 1 View

How would you interpret p-value using Egger's regression test?

Do you believe p-value

20 November 2017 5,225 7 View

Why i can't transfect huh-7 cells with an ad5 adenovirus vector?

06 April 2017 4,301 2 View

Does anyone knows how to clean a silver foil working electrode from any organical traces?

07 January 2016 6,026 6 View

Which is the best method for attaching CeO2 nanoparticles in graphene oxide hydrogel?

I have prepared graphene hydrogel from aqueous dispersion with ascorbate acid.

19 November 2015 8,658 3 View

European funds for research on the topic of the research is "Pescetarian diet in the prevention of breast cancer recurrence", anyone wants to join?

hallo I'm looking for anyone who wants to join an european call for funds. The topic of the research is "Pescetarian diet in the prevention of breast cancer recurrence" Anyone interested? thanks

19 July 2015 2,451 5 View

Range normalization in a semi-structured interview

Hi, I have data from a semi-structured interview called BPASS, my problem is that the coding system for some questions range from 1-5 while for other questions it ranges from 1 to 4. Now I wanted...

19 October 2014 2,645 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View