I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the expression of both the cytokines was seen good.
For this time, i run out of cytoperm/cytofix reagent and i have a patient to be evaluated.
If someone has used any alternative methods of permeabilization for this test is requested to suggest.
Thanks in Advance.