Does anyone have knowledge of ERK phosphorylation by a kinase other than mitogen-activated protein kinase (MEK)?
SGK1 has been shown to phosphorylate ERK2, see http://www.ncbi.nlm.nih.gov/pubmed/19447520
Hey Daniel, do you have access to the full article?
Hi,
The phosphosite plus site shows more sites on ERK2 (autophosphorylation, CK2 etc) with some references.
http://www.phosphosite.org/proteinAction.do?id=832&showAllSites=true
The above mentioned article (sent) is about one of those sites. General trend seems to be phosphorylation on other sites causing autophosphorylation at the TxY motif.
I want to verify, by western blotting, the formation of dimers of a 350 kDa protein (dimer is therefore ~700 kDa). Any information about transference of high molecular weight proteins (I use an...
09 October 2013 3,655 5 View
I'm looking for suggestions to improve the elution step of immunoprecipitations that doesn't involve reducing agents (such as beta-mercaptoethanol). I've tried 0.1 M glycine pH 3, but the yield is...
31 December 2012 1,352 17 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View