Luiz Godoy

3 Questions 4 Answers 0 Followers

Questions related from Luiz Godoy

Analysis of dimerization of high molecular weight proteins by western blotting?

I want to verify, by western blotting, the formation of dimers of a 350 kDa protein (dimer is therefore ~700 kDa). Any information about transference of high molecular weight proteins (I use an...

10 October 2013 3,642 5 View

Non-reducing elution for IP?

I'm looking for suggestions to improve the elution step of immunoprecipitations that doesn't involve reducing agents (such as beta-mercaptoethanol). I've tried 0.1 M glycine pH 3, but the yield is...

01 January 2013 1,333 17 View

Alternative extracellular signal-regulated kinases (ERK) phosphorylation anyone?

Does anyone have knowledge of ERK phosphorylation by a kinase other than mitogen-activated protein kinase (MEK)?

01 January 2013 6,375 3 View