Hello,
I conducted an wheat seed germination bioassay where the seeds were treated with 5,10 and 15% concentration of methanol, ethyl acetate and aqueous extracts of the aerial parts of Hygrophila spinosa.
After 5 and 10 days of treatment, shoot and root lengths of each seedling were recorded. What the data shows is that, the methanol and aqueous extract inhibited the root and shoot growth compared to control but the ethyl acetate extract had highly significant growth stimulatory effect.
Is it normal to have two different extract of the same plant parts to have opposite effects like this?
Thank you for your answer, really appreciate it.
Your effect may be due to the different solvents. But different concentrations of extracts can have opposite effects
Thank you Mr. Pegman for the answer. Do u think I can publish the work as the plant having genuine allelopathic effect? or should i Do the test again using a different method?
The purpose of using a number of solvents to extract active compounds from plants. Is that different solvents dissolve different compounds according to their partition coefficient. In other words, different plant extracts contain different compounds. Some may be growth stimulants and others are inhibitors of growth. But some of them stimulate the growth of branches and inhibits the roots and so. In one of our experiments on wild plant extracts, it was found that some extracts activated the growth of wheat seedlings.
In our experiments. Some extracts in low concentrations stimulated the growth of wheat seedlings and in high concentrations reduced growth. These are normal results and do not fear them.
Just check your results (try again in the same way) if it gives you the same results statistically, publishes your results, there is no problem, you have a good results
Yes, like the others, I too have observed the concentration of the extract to affect the response. In an unpublished project I observed low concentrations (2g/100ml) of Arundo donax aqueous extract stimulate lentil growth, but a higher concentration (10g/100ml) significantly reduce lentil growth. The same plant material exracted with 70% methanol yielded 0% germination. Allelolopathic extracts are complicated mixtures and are not yet well understood. The effect you describe is not surprising. Some commercial herbicides such as 2,4-D exhibit a similar effect based on the application rate. I also agree that the choice of solvent may effect the response.
hi Sayeman,
as the others said, and according to my results, low concentrations did not cause inhibition of growth not only for wheat plants. on the other hand, other plants species were more tolerant to the same high concentrations. that what we called (concentration/species dependence). try to see my file and check my papers about allelopathy.
Regards, Salem
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