Sayeman Islam Niloy

12 Questions 24 Answers 0 Followers

Questions related from Sayeman Islam Niloy

Problem with Fluo-4 calcium imaging?

Hi everyone, I am trying to measure calcium sparks and transients in freshly isolated rat mesenteric smooth muscle cells using Fluo-4 (5 microM). we have a Zeiss LSM 900 confocal microscope that...

02 September 2021 7,440 7 View

No channel activity in Cell-attached patch clamp?

Hello there, We use cell-attached patch clamp for BK channel activity in Vascular smooth muscle cells. Unfortunately 99% of the time I don't see any channel activity even at high command voltage...

10 March 2020 1,374 13 View

Droopy unstable baseline in cell attached patch clamp (Voltage clamp)?

Hi there, Recently I have been facing an issue where I would get a nice gigaseal with resistance hovering around 1.5-16 gigaohm, but when I am recording channel activity, for 2-3 minutes the...

30 January 2020 8,566 5 View

Question about I-V curve in cell-attached patch clamp (voltage clamp)?

Hi there, looking for some help regarding the I-V curve in cell-attached patch clamp (voltage clamp) as I am kinda confused. Does the current (I) in the I-V curve, represent the highest peak...

20 January 2020 9,574 5 View

Question regarding cell-attached patch clamp and BKca channel?

Hi everyone, I have a few questions regarding the cell-attached patch clamp and BKca channel - 1) What I know is cell-attached patch clamp is reversed, that means the holding potential and...

20 January 2020 8,926 5 View

Little help with patch clamp?

I have recently started using patch clamp to measure BKca channel current in neurons (whole brain culture) and in mesenteric vascular smooth muscle cells. But after getiing a gigaohm patch in...

07 November 2019 4,679 5 View

Some help with neonatal rat cardiomyocyte isolation?

Hello everyone, I am trying to isolate cardiomyocytes from a day old rat pups for patch clamping. I use trypsin 0.1% and 50microgram/ml DNAse 1 in HBSS to digest the ventricle pieces. Cells are...

20 August 2019 8,860 4 View

Allelopathic effect of different extracts of the same plant?

Hello, I conducted an wheat seed germination bioassay where the seeds were treated with 5,10 and 15% concentration of methanol, ethyl acetate and aqueous extracts of the aerial parts of Hygrophila...

27 March 2018 2,397 10 View

Should percentages have Standard error of mean?

Hello, I conducted an antiemetic evaluation test on 4 local plants in chicks using the Copper Sulfate-induced chick emesis test. My question is, should the "% of inhibition of emesis" have...

15 March 2018 367 2 View

Any advantage of using Diclofenac as a standard in Membrane stabilization test?

In membrane stabilization of Human red blood cells against heat- induced and hypotonicity-induced hemolysis, is there any advantage of using Diclofenac over other anti-inflammatory drugs? Thanks...

22 October 2017 8,769 0 View

What should i use as control in the control group of mice?

I have 3 extract of the same plant.Aqueous extract - dissolves in salineMethanol & Ethyl acetate extract - Won't dissolve in saline, needs DMSO.Now if i decide to do a experiment using these 3...

16 December 2016 8,640 2 View

Hello, if i use 5% DMSO to dissolve my Aquous, Methanolic and Ethyl acetate extracts for administration in mice, do i have to use 5% DMSO as control?

I am currently working on the anxiolytic effect of Hygrophila Spinosa, i have 3 types of extract of this plant - Aqueous, Methanol and E. Acetate extract.i will be using 3 concentrations...

09 December 2016 5,949 7 View