Hello everyone, may I ask about the purpose of adding ultrapure water after incubation for 30 minutes at 80 degree Celsius (under saponification process of vitamin D?
Secondly, what is the purpose of adding 40% ethanol solution during extraction process of vitamin D? Then, during washing step, the ether layer was washed once with 1M potassium hydroxide, twice with 40% ethanol and lastly once with ultrapure water and I am unsure what is their purpose of washing ether layer with these reagents. I have tried to search online but it does not mention on the purpose.
I am looking forward to answers from everyone, thank you in advance!
I believe that your sample preparation (for HPLC ?) is rather old-fashioned. Modern methods use saponification and hexane extraction, like the method described in Article Convenient Analysis of Vitamin D in Cheese and Other Food Ma...
Jos Wielders Dear sir, yes the sample preparation is for HPLC analysis. Thank you for the article, will read it up :)
Please look at the following below attached files which may help you in your analysis.
Thanks
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