How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Can anyone direct me to a paper or chapter that discusses calculating sample size in a self-selecting survey study?

Our study is investigating clinical knowledge in a specific area in healthcare workers at a large metropolitan hospital. Participants will self-select to complete the survey via email invitation...

11 January 2021 7,934 2 View

How to get return loss below -10dB for monopole antenna which has very less or no ground plane (or directly interfaced with sma connector) ?

I am designing a monopole antenna For 59MHz frequency . The lamda length is 5m. I tried for lamda/2 which is directly interfaced to sma connector. Couldn't get proper return loss.( According to...

11 March 2020 4,885 2 View

Can anyone recommend a stain to identify mast cells/eosinophilic granular cells (EGC) in fish?

Hi, I was wondering if someone can recommend a stain where fish EGCs or mast cells show significant metachromasia. Toluidine blue did not work in my fish species. I did find recommendations on...

04 March 2020 7,817 1 View

Alpha/betha TCR detection in naive TCs without activation?

Hello together, I want to see differences in TCR expression in naive CD4/CD8 TCs. The problem is, if I would stain the TCR with soluble antibodies to check via FACS, this would lead to a weak...

23 November 2019 1,430 1 View

Questions about RNA extraction?

Hello, I have a problem with my gene expression results. The results of my endogenous gene are oscillating between samples, generating false negative results. It takes about 12 hours after sample...

08 October 2019 569 3 View

Why did I get RMSD plot with 10ns if the MD was 100ns trajectory?

I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...

04 September 2019 3,669 6 View

Doubt about secondary antibody to immunohistochemistry?

I bought a secondary antibody to use for immunohistochemistry, but the manufacturer says the antibody is only validated for Western Blotting, not Immuno. Because of this, I don't think which...

26 August 2019 5,356 7 View

High background on stain free blot after transfer and its complications for protein normalization(TGX Stainfree Biorad Gel)?

Hey, I have a question regarding the stain free blot technology of Biorad. After electroforesis I take a picture ("Stain-free gel") for activation of the trihalo-compound. Next I transfer the gel...

12 August 2019 7,734 8 View

What are the strongest and most relevant indicators that have lead patients to suffer from Muscle Tension Dysphonia? Are robust orators more at risk?

i am working on research on this topic. MT Dysphonia is my diagnosis. I am a teacher who is trying to overcome the short and long term effects of this silent disorder. I was first diagnosed in...

06 February 2019 4,656 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View