i ask for someone who work with AGS cells, about the best growth conditions of this cells, the best medium, protocols, all tips that can help
thank you
I use to culture AGS cells in 10%FBS+RPMI-1640. Doubling time is almost 48 hours. Trypsinization (0.25% trypsin; 0.05% EDTA) 90-120 seconds. Culture type: Adherent. Storage: Liquid nitrogen vapor phase
Thank you Muhammad
you have a special tips to work with this sort of cells ?
Hi,
I attached for you one complete protocol for AGS cells with medium formulation, tips for subculturing, etc.
Hope it will help.
I culture AGS cells in 10%FBS+ham's F-12. And the serum is from Gibco. I have known RPMI-1640 is ok for AGS but I haven't used it.
Hope it work.
Dear,
I routinely culture AGS cells for my experimental studies. I found the cells grow well in RPMI1640, 10% FBS, 1% PC/SM antibiotics.
Best of luck
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