Dear All,
I am looking for Rhizobium rhizogenes, strain ATCC 15834.
I got culture from Spanish cell bank, but cells don’t look vital.
No growth after 5xON on LB plate.
Agrobacterium rhizogenes strain K599 is a causative agent of hairy root disease which induces hairy root formation
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
I regress X to Y: ,direct effect (c) M: mediator: I regress X to M (a), M to Y (b) Total effect = c + a*b now i introduce a moderator effect between X and Y How i calculate the total effect with...
08 March 2024 9,816 1 View
Explanation: Let I have downloaded 25,000 from Scopus database. I have all DOI in a csv file. Now what is the procedure to get RG statistics (read, citation, recommendation etc) in excel file. I...
02 December 2023 9,744 1 View
hello, i am intersted in the cause of getting higher concentration of RNA when using the acid phenol chlorofom extraction to the qiagen kit.. i am extracing RNA from cells and i tried both method...
18 September 2023 6,480 0 View
Becoming part of politics by withdrawing invitations to Russia, Belarus and Iran , Nobel Prize committee becomes then part of politics, what consequences of this decision ? do will have two (2)...
01 September 2023 542 5 View
I will perform gene expression for mir27a and mir155 so I need to check my primers by any suitable software thank you
21 August 2023 9,964 4 View
hello, I am curious about the use of RBS designer from the website: https://salislab.net/software/ how is it possible to use it for designing new RBS to overexpress protein in e.coli? thank you...
27 July 2023 6,193 2 View
hello, I have a protein with a molecular weight of 100 kDa and the concentration is 1pmol/ul. how is possible to convert the protein concentration to ng/ul? thank you for your help.
19 July 2023 5,897 1 View
What is chlorophyll a fluorescence?
14 July 2023 4,396 3 View
What is the most relevant measurement scale that could be used to assess secondary school teachers' performance in the Moroccan context ? thank you in advance
15 June 2023 7,870 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View