In Agilent two-color microarray experiment how to separate data from Cy5 and Cy3 in a single sample using GeneSpringGX and how to select parameter that it can see only one color at a time?
Hi, I am trying to screen cells based on CRISPR-cas9 KO of my target gene. The clones were derived from a single cell after CRISPR-cas9 KO. It would be very robust to extract DNA from each clone...
03 April 2019 6,971 5 View
Does anyone know the positive control cells for stem cell marker CD133.
02 March 2017 5,644 0 View
I need to analyses some pathways connected with cancer stem cell related genes. Waiting for your answers. Thank you
07 August 2015 754 4 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....
21 February 2021 8,609 1 View
What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...
21 February 2021 1,831 4 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...
17 February 2021 4,118 1 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...
16 February 2021 8,731 1 View
I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...
09 February 2021 2,648 3 View
My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...
08 February 2021 8,628 3 View
I would like to read about your experiences in the measurement of the color of liquid samples (wine, oil, or whatever) using a glass cuvette to calculate L* a* and b* parameters with the...
08 February 2021 6,619 4 View