Agar diffusion assay

Jannatul firdous Siddique @Jannatul-Siddique

05 March 2017 1 4K Report

what are the minute parameters followed in agar diffusion assay? such as pH, adding any enzyme aor microfiltration needed? if possible give reference where exact quantities are mentioned in each step.

Majid Pirooz

· Read the following refs

Jorgensen, J. H., & Turnidge, J. D. (2015). Susceptibility test methods: dilution and disk diffusion methods. In Manual of Clinical Microbiology, Eleventh Edition (pp. 1253-1273). American Society of Microbiology.

Bonev, B., Hooper, J., & Parisot, J. (2008). Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. Journal of antimicrobial chemotherapy, 61(6), 1295-1301.

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