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Questions related from Jannatul firdous Siddique
the supernant contains all the low molecular proteins dissolved, tried it with ammonium sulfate ppt, till it reached saturation point, even after that the protein can't be extracted. please suggest.
25 January 2018 1,764 0 View
I have checked for activity with 30%, 60%, 80% precipitated pellet suspened in buffer, it didn't show any zone, whereas the treated supernatant such as 30%, 60%, 80% keep on showing zone against...
29 December 2017 5,290 0 View
what are the minute parameters followed in agar diffusion assay? such as pH, adding any enzyme aor microfiltration needed? if possible give reference where exact quantities are mentioned in each...
05 March 2017 3,807 1 View
storage temp? distilled water is correct to use, or particularly any buffer is needed?
02 March 2017 9,940 2 View
for low molecular, sensitive, generally shows activity below pH 5. for this kind of peptide(made up of few amino acids), which buffer will be the best? and how to choose? In case of ammonium...
01 January 1970 9,775 2 View
Kindly , suggest where to learn about data interpretation for Mass spectrometry analysis? Note: LCMS for peptide or protein identification using mass-to-charge ratio (m/z) of one or more molecules...
01 January 1970 9,826 2 View
If I have 3kDa ultratfiltration membrane and I have a mixture of unknown peptides, molecular weight ranging from 5-25kDa. Kindly tell me how the separations will take place and how the peptides...
01 January 1970 5,552 2 View
