03 September 2015 5 9K Report

I currently plan to use pichia to do heterologous expression of a gene from white rot fungi.

Unfortunatelt, I only have the gDNA sequence of the gene. Can I use it for ligation to pPICZ alpha and more importantly, will it be efficiently spliced once the vector is transformed to pichia?

Anyone with experience of using gDNA to express extracellular protein in pichia system? Or is the use of cDNA mandatory?

Any suggestion or opinion will be welcomed. Thanks! :)

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