Hello,

Seems like you have a concentration or contamination problem here, do you have a picture of the gel including your bands? Did you get no peak or multiple peaks in your nanodrop readings?

Clean the nanodrop prob with ethanol (70%), if any contamination occurs that might shadow the real reading of your DNA sample,

If I see the nanodrop result or your gel photo, I can say more certain answer,

If you are unable to measure the DNA concentration of your purified sample using a nanodrop spectrophotometer but can visualize it on a gel, there could be a few potential reasons for this discrepancy:

To address these issues, you can try the following steps:

• Ensure that the purified DNA sample is free from contaminants by performing additional purification steps or using alternative purification methods.
• Dilute the DNA sample appropriately to fall within the detection range of the nanodrop spectrophotometer.
• Consider using an alternative method, such as a fluorometric assay or a different spectrophotometer, to measure the DNA concentration.
• Verify that the nanodrop spectrophotometer is calibrated and functioning properly. Consult the instrument manual or contact the manufacturer for guidance on calibration procedures.

If the issue persists or you need more accurate DNA concentration measurements, it may be helpful to consult with experienced researchers in your lab or seek guidance from technical support or experts familiar with the specific equipment and protocols you are using.

Hi, I had similar experience. The concentration as measured by nanodrop decreased about 100-times, although on the gel it looked about the same (I loaded twice higher volume of samples after purification).

The large bands are gone, but in general, the samples look the same.