Umut Karatekin

3 Questions 1 Answers 0 Followers

Questions related from Umut Karatekin

PCR Sample Purification, what is the difference between KOH and NaOH?

I have 2 protocols for PCR sample purification. One uses Sodium for salt and other is using Potassium for salt. What is the reason for using different chemicals for salt in purification protocols....

07 September 2023 9,128 3 View

Can a gene be cloned along with the intron?

Hello, dear researchers. I am interested in a gene in Pombe. The gene contains 2 introns. I want to overexpress this gene in Pombe. Can I amplify and clone the gene by PCR or do I need to do cDNA...

05 September 2023 4,525 4 View

After purification from agarose gel, DNA concentration cannot be measured in the nanodrop, but can be visualized in the gel. What could be the reason?

Hello dear researchers. After gel purification of the plasmid cut with restriction endonucleases, I cannot take measurements on a nanodrop. However, when I load the sample on an agarose gel, I can...

19 May 2023 860 3 View