After microirradiation, what temperature I should use for incubating HeLa & U2OS cells before fixation?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Chetan Rawal

For a short kinetics its fine to incubate at RT lets say 5 or 10min after IR. But if you want to keep for long time after irradiation better to incubate at 37. :) And keep the same treatment for all the experiments.

Aman Sharma

For longer intervals/assessment, incubation with 37C is preferable.