I am working on exosome isolation from conditioned media. I am using MC3T3-E1 cell line as the source. Before going for isolation, I used to keep the cells with serum free media for 16 hrs. Any suggestions if I can use these cells again for culture or any other experiments?
Thanks in advance.
Dear Sasmita, this is dependent from your aim. Serum-free medium led to starvation, but cells eventually can "recover" after addition of new serum.
This approach can serve as a good model for cell recovering from starvation.
My best wishes!
I suggest you to compare epigenetic profile cells before and after starvation. This can serve as a good staring point for further investigation.
Thank you Taras Pasternak for your valuable suggestion! I will surely plan to work on it. However, after keeping cells in serum free media, I again added normal growth media and the cells are growing fine.But yes, looking into the epigenetic changes will be interesting.
Yes, sure, 16 h is not enough to get irreversible epigenetic changes in chromatin. Longer starvation (24-36 h) will have more visible effect.
But,I am sure,even after 16 h some changes may happens.
My best wishes!
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