After extracting DNA and storing it in low salt TE buffer. How long can the DNA last?

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Breaking pupal diapause in saturniids (Emperor moths)?

I am trying to rear saturniid moths in the lab and they keep diapausing at the pupal stage. They stick at this level for months on and am unable to raise a colony. Anyone knows how I can break the...

09 October 2017 3,376 3 View

Synchronizing mating and breaking diapause in saturniid moths?

We are trying to maintain a colony of saturniid moths collected from the field. However, we are experiencing problems with mating. The males and females emerge at different times. These moths only...

10 November 2016 4,311 4 View

Can I use primers that were used with a taqman probe for qpcr using sybr green?

I want to quantify RNA plant viruses using RT-qPCR. I have some primers that I found in a publication. The primers were used witha taqman probe. However, I have SYBR green mastermix in the lab. Is...

07 August 2015 8,832 5 View

How can I perform plant sample sterilization before RNA extraction?

I am looking for alternative hosts of a maize virus. I want to sterilize the leaf samples of the suspected hosts before total RNA extraction, since I am working with an RNA virus. I just want to...

09 October 2014 1,634 3 View

What does this error message mean and what can I do?

I tried to submit sequences to the GenBank, but got an error message indicating that there are non-IUPAC residues in the sequence. What does this mean and what can I do?

08 September 2014 6,847 3 View

Can someone help with DNA sequencing and analysis?

Am working on a plant RNA virus. I did RT-PCR then purified the PCR products and sent them for sequencing. Note: I did not excise gel. However am not sure whethet the sequences I got are ok. The...

07 August 2014 790 5 View

Any advice for sampling maize and wild grasses for viral RNA extraction?

I want to collect leaves from maize and wild grasses from the field but am not sure what method will be best to maintain the integrity of the viral RNA for extraction and RT-PCR. Should I use FTA...

31 December 2013 2,032 3 View

DNA quantification

I am working with a plant pathogen that cannot be cultured, I want to determine the difference in concentration of the pathogen in different parts of the plant for the purpose of standardizing my...

06 July 2012 2,560 8 View

Quantifying DNA using gel photos

I would like to quantify DNA using gel photos instead of extracting the DNA from the gel. Please help if you have information on a software that I can use to do this.

02 March 2012 6,879 9 View

Negative control turning positive in Loop Mediated Isothermal Amplification of DNA (LAMP).

I am using LAMP to amplify 16SrRNA for detection of phytoplasma in napier grass. In the past week, the negative control has been appearing positive on agarose gel electrophoresis. I even tried...

02 March 2012 457 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View