After extracting DNA and storing it in low salt TE buffer. How long can the DNA last?

More Elizabeth Siago Kusia's questions See All

Breaking pupal diapause in saturniids (Emperor moths)?

I am trying to rear saturniid moths in the lab and they keep diapausing at the pupal stage. They stick at this level for months on and am unable to raise a colony. Anyone knows how I can break the...

09 October 2017 3,457 3 View

Synchronizing mating and breaking diapause in saturniid moths?

We are trying to maintain a colony of saturniid moths collected from the field. However, we are experiencing problems with mating. The males and females emerge at different times. These moths only...

10 November 2016 4,398 4 View

Can I use primers that were used with a taqman probe for qpcr using sybr green?

I want to quantify RNA plant viruses using RT-qPCR. I have some primers that I found in a publication. The primers were used witha taqman probe. However, I have SYBR green mastermix in the lab. Is...

07 August 2015 8,919 5 View

How can I perform plant sample sterilization before RNA extraction?

I am looking for alternative hosts of a maize virus. I want to sterilize the leaf samples of the suspected hosts before total RNA extraction, since I am working with an RNA virus. I just want to...

09 October 2014 1,679 3 View

What does this error message mean and what can I do?

I tried to submit sequences to the GenBank, but got an error message indicating that there are non-IUPAC residues in the sequence. What does this mean and what can I do?

08 September 2014 6,911 3 View

Can someone help with DNA sequencing and analysis?

Am working on a plant RNA virus. I did RT-PCR then purified the PCR products and sent them for sequencing. Note: I did not excise gel. However am not sure whethet the sequences I got are ok. The...

07 August 2014 838 5 View

Any advice for sampling maize and wild grasses for viral RNA extraction?

I want to collect leaves from maize and wild grasses from the field but am not sure what method will be best to maintain the integrity of the viral RNA for extraction and RT-PCR. Should I use FTA...

31 December 2013 2,081 3 View

DNA quantification

I am working with a plant pathogen that cannot be cultured, I want to determine the difference in concentration of the pathogen in different parts of the plant for the purpose of standardizing my...

06 July 2012 2,602 8 View

Quantifying DNA using gel photos

I would like to quantify DNA using gel photos instead of extracting the DNA from the gel. Please help if you have information on a software that I can use to do this.

02 March 2012 6,927 9 View

Negative control turning positive in Loop Mediated Isothermal Amplification of DNA (LAMP).

I am using LAMP to amplify 16SrRNA for detection of phytoplasma in napier grass. In the past week, the negative control has been appearing positive on agarose gel electrophoresis. I even tried...

02 March 2012 507 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View