Hi everyone, I’m trying to troubleshoot an issue I observed in my agarose gel image and would really appreciate your insights.
In the gel photo, the first lane is a DNA ladder, the second and third lanes are two different inserts, and the fourth lane is the vector. I took this picture before ligation to check the quantity of each DNA component.
In the fourth lane (the vector), I observed significant smearing. I'm wondering what might have caused this and how I can fix or prevent it.
Here are the details of the experiment:
Any suggestions or explanations would be greatly appreciated. Also, apologies for any awkward English—it's not my first language.