Hi all, this is my first-time culturing lymphoma cell line.
I obtained a frozen vial of EL4 (in the background of B6 mice) lymphoma cell line, and I am the only one culturing this line in the lab.
I checked on ATCC that it should be a suspension cell line, but I noticed that over half of the cells have become adherent on the next day, is it normal?
https://www.clsgmbh.de/pdf/el4.il-2.pdf
If the culture is in suspension, it should grow in suspension and not adhere. try to contact those who gave you the culture (it is possible that this is not a pure culture, that is, there are other types of cells or damaged). try growing cells on low-adhesion plates or dishes to avoid adhesion.
- You should check the media, "DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)".
- You have to check the type of cells from the source.
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