hello everyone, do you have any advice on advice for Agar disk-diffusion yeast method?
like, medium agar to use and how many microlitres to use to wet the disk-diffusion?
thank you all for your help
hello, thank you for your reply.
i know you use microdilution according to EUCAST but i wanted to do some preliminary tests with disk diffusion. To avoid waste and time.
Since these are antimicrobial trials with new drugs I wanted to know how much to use to put it on the disk
I am not an expert in this field, but I am very interested and have researched to find an answer. Could you please review the response below to see if it is correct?
For the agar disk-diffusion yeast method, it is essential to choose an appropriate medium and optimize the volume of the antimicrobial solution used to wet the disk. Based on the articles reviewed, RPMI agar containing 2% glucose (RPG) and Casitone agar (CAS) are highly recommended, as they support robust growth and provide high agreement with reference MICs [1]. A suitable volume to wet the disk typically ranges from 10 to 20 microliters, ensuring even distribution without oversaturation [2]. Variability in inoculum size and reading times can significantly impact results, so standardization and precise timing are crucial [2][3].
Reference
[1] Pfaller, M., Messer, S., Karlsson, A., & Bolmström, A. (1998). Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media. Journal of Clinical Microbiology, 36, 2586 - 2589.
[2] Brass, C., Shainhouse, J., & Stevens, D. (1979). Variability of Agar Dilution-Replicator Method of Yeast Susceptibility Testing. Antimicrobial Agents and Chemotherapy, 15, 763 - 768.
[3] Edelstein, P., & Edelstein, M. (1991). Comparison of different agars used in the formulation of buffered charcoal yeast extract medium. Journal of Clinical Microbiology, 29, 190 - 191.
I received some assistance from tlooto.com for this response.
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