Greetings everyone,

I am looking for an assay suitable to detect atleast, 500nM to 1mM ATP hydrolysis. My Kinase has a very weak activity. The signal should be good enough (also must be duplicated) to have good signal/noise and Z´values.

I want to use the assay for Inhibitor screening. Then the goal is to use the compound in cell-based assays and do structural studies.

ADP-Glo assay is pretty expensive.Cost factor comes into play as I want to screen atleast 5K compounds.

In the same cost of 1000 rxns of ADP-Glo assay I can do 10,000 rxns of Kinase Glo assay.

Does anyone have experience with Kinase Glo plus assay (linear upto 100uM ATP)? and could please recommend its suitability.

Or alternatively any other suitable assay for the above mentioned requirement.

PS- Malachite green assay (MLG) is not suitable.

With another kinase, I used MLG assay and screened 16K compounds. MLG dye, I made myself and it is cheap.

Thanks and best wishes,

Saif