Addition of reducing agent to media?

13 June 2018 1 9K Report

Hello All,

I am trying to add a reducing agent (L-Cysteine or Sodium Thioglycolate) to my media. Does it matter if I add it before or after autoclaving? I read that Sodium Thioglycolate needs to be heat-activated.

Saif Hosam Raheem

https://www.sciencedirect.com/science/article/pii/S0167701211000248

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