Adding to my skill set

David Tacha @David-Tacha

01 January 1970 1 5K Report

I would like to add another skill set to my profile. Currently I only see one.

Immunohistochemistry

I would like to add Pathology as this is the big portion of my publications

David Tacha

Thank you so much. It worked!

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Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

How do you add multiple nodes or cutting planes in Abaqus?

Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...

20 June 2024 892 3 View

How do you add radial spring elements in Abaqus?

Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...

17 June 2024 8,165 7 View

Given that Mesozoic placentals were largely arboreal (with fingers), do you know of any studies that illustrate the finger-to-hoof transition?

I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?

06 June 2024 5,182 2 View

How reliable is ninhydrin test in solid-phase peptide synthesis?

Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...

21 May 2024 9,035 0 View

How does one generate a normalized TCSPC curve from actual photon counts versus time?

Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...

20 May 2024 4,277 0 View

Problem when running Spin-Orbit Coupling using CASTEP. What is nlpot_prepare_precon_ks?

I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...

19 May 2024 4,236 5 View

A paper on a fossil lycopod?

Dear all, Does anybody have a pdf of this paper? Wood, G.R. and Beeston, J.W., 1986. A late Permian lycopod cone Skilliostrobus sp. cf. S. australis Ash 1979 from Queensland. Geological Survey of...

07 August 2024 4,857 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Merging profiles after change in name due to marriage?

Hello- I have two profiles in ResearchGate. One under my married name of Kristina Secnik and one under my married name of Kristina S Boye or Kristina Boye. How do I merge my publications so that...

29 July 2024 4,426 1 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View

Need publications on Equity Multiplier and Bank Performance ?

I need publications on Equity multiplier

24 July 2024 6,071 1 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

I accidentally removed 5 publications a few minutes ago. How can I add them again to my profile?

23 July 2024 9,145 2 View