Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Which points I have to take in mind to mutate a plasmid that grow in Neb stable strain?

Several kits that allow the introduction of point mutations in plasmids say to transform mutated plasmids in DH5alpha bacteria. I have a plasmid with many repetitions that need stable strains to...

08 November 2020 4,250 2 View

Did you have any problem for staining nuclear phosphorylated proteins in hippocampal neurones?

I was wondering if there may be a problem of permeabilisation of cells. I am working on mice hippocampus (pups) and I need to make a staining for a phoshoprilated nuclear protein. Since the...

17 September 2020 2,000 3 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How many researchers are interested in attending web communications in the field of Sustainable Planning?

17 June 2020 4,753 74 View

What happens to non-"preclincial essential research" (non Covid19) in Covid times?

Are you askiing what researchers are doing in Covid time especially those that work with animal? I started a survey to collect information (anonymous) to see whether there are alternatives to...

02 April 2020 9,596 3 View

Generic DC/HYBRID grid-connected microgrid available in simulink?

I'm looking for a microgrid simulation model written in simulink in order to implement my simulation data and test my Energy Management System algorithm. The microgrid I am looking forward is a...

24 October 2019 3,541 4 View

Is there an investigation about the real world implementation of decision making algorithms in smart home/microgrids EMS microcontrollers?

Implementation of stochastic and probabilistic based algorithm is a very tricky task especially in energy management systems microcontrollers. I'm looking for a research paper which talks about...

30 July 2019 5,947 1 View

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

24 June 2019 3,133 2 View

UDF function suggestion for my case study?

Good morning Everyone, I need a suggestion for my Master thesis study case. I want to simulate the behaviour of a thermal actuator located in a thermostatic mixing valve: I actually have a...

04 December 2018 9,582 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View