We recently in our lab took PDX model and decided to culture cancer cells from that. After digestion and culturing we found that most of our mixed cells were fibroblast instead of tumor cells. Is that normal situation and does anyone know how to get rid of most fibroblasts??
And if we used those cell mixtures to do test I was so afriad that the results might not be what we wanted.
It depends on the goals of your research - I'm not sure fibroblasts would negatively affect any results. There are ways to filter out fibroblasts (cell isolation protocols are out there in the literature) but otherwise it is pretty common for fibroblasts to be present in tumors. Look at some of the common xenografts (http://altogenlabs.com/xenograft-models/) and the associated research, which should help you identify any kind of follow-up issues that might arise.
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