Fei Mao

4 Questions 3 Answers 0 Followers

Questions related from Fei Mao

Has anyone done colon cancer cell 3D culture before?

I am recently doing PDX model but find serious fibroblast cells contamination. Can anyone share experience on that one?

28 October 2017 4,647 2 View

About tumor xenograft--We cultured primary tumor tissue and found most of the cells were fibroblast?

We recently in our lab took PDX model and decided to culture cancer cells from that. After digestion and culturing we found that most of our mixed cells were fibroblast instead of tumor cells. Is...

26 October 2017 1,488 1 View

How long duration of starvation could cause tumor cell death?

I am recently culturing lung cancer tumor cells and it's kind of bothering me that after deletion of FBS for like 4-5 days, the cells are still growing healthily in the dish. I have little...

02 October 2017 6,760 3 View

How to differentiante different parts of the brain exactly?? Do different neurons have different markers?

We are right now working on brain tissues of the mice brain. And we are wondering after we dissect different parts of the brain, how should we know eaxctly which part is which? By immunostaining...

23 May 2017 2,877 2 View