i have protein sample for western blot .the final volume are 200ul.
protein concentration are 1.5ug/ul.
now my question is how much ul should i add per well?
thank you
Hi Ikram Ullah
To determine the volume of protein sample to add per well in a Western blot, you need to consider the desired amount of protein to load and the concentration of the protein sample.
In your case, you have a protein concentration of 1.5 μg/μl, and the final volume of your protein sample is 200 μl. To calculate the volume of protein sample to add per well, you can follow these steps:
Therefore, you should add approximately 13.33 μl of your protein sample per well if you want to load 20 μg of protein, based on the given concentration of 1.5 μg/μl and a final volume of 200 μl. Adjust the volume accordingly if you have different protein loading requirements or if you are using multiple wells.
Remember to consider any additional volumes required for loading buffers, markers, or other components of your Western blot protocol.
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