I have seen in many papers for eg. written like that "The Pairwise distances of the ITS region between species of the ‘glaseri’ group". Below diagonal: percentage similarity; above diagonal: total character differences. I know only how to prepare Below diagonal: percentage similarity; but unaware about how to prepare above diagonal: total character differences. Can anyone please provide me the details of how to prepare this in pairwise alignment via mega. I have attached a file also to make my question more clear so that you could get my question what I actually want.
I used MEGA 5 for the screen shot attached, but MEGA 6 is probably the same for this function. I don't see a way to get both values at once, so you need to copy/paste the values, or if your matrix is huge invert one of them with an automated method.
In Mega 6 and 7 the process is the same. You should calculate the "percentage" similarity (p or k2p or other model) and No. of differences in two step and merge the two matrix.
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