How to deal with computed negative inflows in dams ?

This question aims to explore, from the part of expert colleagues, some methods that can be used to adjust the negative inflows calculated for the dams.

14 December 2019 1,486 3 View

How to choose reviewers for your submission ?

Most of journals require for paper submission to suggest three or four reviewers. How to choose them ? Is it among the authors of the papers cited in your manuscript, among the members of the...

09 September 2019 8,943 2 View

What is the best pipeline design software?

Hello i am looking for a software which can be used to design pipeline for a heating system. I need it to study the stress in pipes etc... Any software which could be tested with a free version...

28 March 2018 3,778 3 View

About the Protein extraction and purification?

Dear Researcher Please, If that possible to extract and purify a protein from SDS page Gel staining with coomassie Bleu. Many thanks

11 January 2017 1,049 4 View

Does anyone measured composite layers engineering constants using DIC for SHPB specimens?

26 December 2014 3,403 2 View

How can I analyze cell staining images using Fiji / ImageJ?

I have several fluorescent microscopy images for cell staining using three channels. Can you please suggest some methods to analyze these images?

05 May 2014 4,270 2 View

Can anyone help with MTT assay results?

I applied a MTT assay for A549 cell lines treated with different concentrations of Aluminium Oxide, I got the data from the plate reader, but I can't decide if my results are good or not ? And is...

30 April 2014 8,508 6 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

Which compounds might cross-react with cortisol in Elisa kit?

Hi, I'm analyzing cortisol in post smolt salmon with two elisa kits. The first is made for human saliva (Fairly cheap) and the second one for fish (three times the price). I was hoping to get...

21 January 2021 6,874 1 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View