About a progesteron kits?

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Urea first hyperpolarizability calculation with gaussian?

have calculated the hyperpolarizability of molecule urea with gaussian in perpus of comparing with some organic compounds using the keyword polar=dcshg with 1064nm as frequency but i get an extra...

23 April 2023 1,023 0 View

How to implement in Matlab the Fourier coefficients of A. R. Stokes 1948 ?

Hi, Please, I would like to know how to implement in Matlab the real and imaginary parts (Gr and Gi) of the Fourier transform given in equations 7 of the paper of Stokes 1948 (A Numerical...

30 November 2022 6,129 2 View

Can we find datasets providing us with real-time data for a given period and whose information is relevant for a case study ?

Seeks for analysis reasons to use more recent datasets possible and why not in real time ?

24 February 2022 331 6 View

About Casa system?

Dear all In your opinion what is the best casa system company? Best regards

10 November 2021 8,775 0 View

How to use quantitative research method design in an unemployment research work?

The question is about contributions of research methods that help to unravel or tackle social or political issues in Ghana

03 October 2021 7,683 5 View

How to explain the strengthening mechanism at high strain rates for GFRP cross-ply composites?

Hi, The ultimate stress of certain GFRP cross ply laminates increases with strain rate increasing . What would be the strengthening mechanism ?

21 June 2021 6,300 1 View

What are the main characteristics and areas of use of GRE cross-ply laminates ?

Please, could you provide any useful references that address the pros and the cons of Glass fiber reinforced epoxy cross-ply laminates with main applications especially in the marine structures...

14 July 2020 550 0 View

How to deal with computed negative inflows in dams ?

This question aims to explore, from the part of expert colleagues, some methods that can be used to adjust the negative inflows calculated for the dams.

14 December 2019 1,663 3 View

How to choose reviewers for your submission ?

Most of journals require for paper submission to suggest three or four reviewers. How to choose them ? Is it among the authors of the papers cited in your manuscript, among the members of the...

09 September 2019 9,091 2 View

There is a defferent beteween the stance and swing phase in the picture beloew, witch picture is correct ?

There is a différent beteween the stance phase and swing phase in the two picture.

08 January 2019 8,793 1 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

If you have a missing trapezoid in total AUC for salivary cortisol data, is this representative of the cortisol changes to estimate sleep changes?

Participants completed 6 saliva samples throughout the day to capture changes in cortisol and melatonin from waking until just before sleep. To achieve total AUC, 5 trapezoids between samples 1-6...

27 May 2024 6,842 0 View

Treatment protocol for SRSE - what works best?

As we know the devastating outcome in Children with SRSE looking for further insights from researchers across the world as what they are trying to get better outcomes for these Children Is Early...

11 May 2024 4,713 0 View

What is the good dilution factor for mouse serum in ELISA?

I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.

28 April 2024 8,922 2 View

Cytokines production in HEK293T cells?

Hello dear researchers,i have transduced my HEK293T cells with IL-2 and then puromycin screening was done, the cells were showing good egfp flourescence ,but then due to some reasons I have to...

14 April 2024 7,285 2 View

US-based labs for hair cortisol analysis?

We are looking for labs based in the US that assess cortisol in human hair samples. We used to send samples to Mark Laudenslager's lab at the University of Colorado for analysis. We saw another...

29 February 2024 3,225 0 View

What is the better practice for adding IL2 to cell culture media?

I am working on a project in which we obtain lymphocytes through Ficoll density gradient separation and then culture them for ~3 weeks. Naturally, we add IL2 to our cell culture media. There are...

28 February 2024 1,276 1 View

How purification exosome from primary human NK cells and CD4+ T cell ?

Greetings I possess a keen interest in exploring the potential of primary human CD4+ T cell and NK cells-derived extracellular vesicles. Currently, I am in the process of formulating a research...

25 February 2024 3,422 0 View

Which TH2 cytokine is easier to detect in an antigen-specific T cell assay by using flow cytometry intracellular cytokine staining?

Hello, I am planning an experiment to detect human antigen-specific TH2 T cells and would like you to learn from your previous experience. By using flow and ICS, in a time frame of 6h or overnight...

25 February 2024 9,099 2 View

Which is the correct answer for the immunology question in description?

While studying immunology i came across the following question. The following figure shows the result of flow cytometry of human blood cells. The cells were stained with FITC-conjugated rabbit...

19 February 2024 3,148 4 View