While studying immunology i came across the following question.
The following figure shows the result of flow cytometry of human blood cells. The cells were stained with FITC-conjugated rabbit anti-human IL-2 receptor a subunit (y axis) and conjugated mouse anti-human IL-2 receptor y subunit (x-axis). Which quadrant shows cells expressing the medium affinity receptor?
MY ANSWER : upper right: HIGH affinity, UL - LOW affinity, LR - INTERMIDIATE/ MEDIUM affinity, LL -cells that do not express IL-2.
Book answer: upper right: medium affinity, UL - intermediate affinity, LR - low affinity, LL -cells that do not express IL-2.
if the book answer is correct, please explain it.
the figure in the question is nothing but a square which is divided into 4 quadrants with black circles in each quadrant. nothing else was mentioned in the figure.
I don't fully know if I understood the question correctly, cause I didn't saw the graph you're referring to, but I guess is one like the following.
Each quadrant indicate the presence (positive (+) or negative (-)) of the staining on the cells you are studying.
can you kindly show the graph? That will aid understanding of the question.