recently I got some problems with my spreading of agar plates.
1. The first plate on 7.8 got nothing in PCR (we did a clone transformation) but I think the major problem is from ligation. However, the spreading also looks not very good. My supervisor says it may be due to the glass I used to spread was too hot and deactivated the ampicillin. Is that reasonable?
2. The second one on 7.25 is pretty confused to me. I think it is because it was too wet so that the E.coli can move freely on the plate. consequently, I did not get any single colony. But I am not very sure about the reason. I want to see what you think about this. Any reply will be very appreciated.
3. BTW, do you have any tricks or suggestions on spreading a plate?
4. how do you decide the temperature to add ampicillin? to prevent it from degrading and prevent the media from solidifying.
Thank you!
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